Adapting a published method to your reagents and equipment

1 · Learn the move · Context + audience

A published method is written for their bench: their kit, their rotor, their buffer stocks, their sample amounts. The model cannot see yours, so an adaptation is only as good as the inventory you hand it - which reagents you actually have, what your centrifuge tops out at, what your starting material is. Given that context, the useful output is a draft protocol with every departure from the published version flagged as a DEVIATION, each with a note on what it might change. What it is not: a validated method. The model drafts the adaptation and lists what a pilot run must confirm; whether the adaptation works is something the pilot decides, not the text.

Here is a published method: [paste]. Here is my bench: [reagents on hand with catalog numbers, instruments with limits, starting material and amount]. Draft the adapted protocol. Flag every departure from the published version as DEVIATION with what it might affect. Anything you need that I did not state, write [NEEDS: ...] - do not assume my stocks. End with what a pilot run must confirm before I commit real samples.